Trichomonas vaginalis is an anaerobic, flagellated protozoan parasite and the causative agent of trichomoniasis. It is the most common pathogenic protozoan infection of humans in industrialized countries. Transmission usually occurs via direct, skin-to-skin contact with an infected individual, most often through vaginal intercourse. Usually treatments consist of metronidazole and tinidazole.
Candida albicans is a dimorphic fungus that grows both as yeast and filamentous cells and one of the few species of the Candida genus that cause the infection candidiasis in humans. It commonly occurs on mucous membranes in the mouth or vagina.
The Trichomonas/Candida Combo (Vaginal discharge) Rapid test is a qualitative lateral flow immunoassay for the detection of Trichomonas vaginalis and/or Candida albicans antigens in female vaginal discharge samples. The membrane is pre-coated with monoclonal antibodies against Trichomonas vaginalis and/or Candida albicans antigens on the test line regions. During testing, the sample reacts with the particles coated with anti-Trichomonas vaginalis and/or Candida albicans antibodies which were pre-dried on the test strip. The mixture moves upward on the membrane by capillary action. In the case of a positive result the specific antibodies present on the membrane will react with the mixture conjugates and generate one or two coloured lines. A green coloured line always appears in the control line and serves as verification that sufficient volume was added, that proper flow was obtained and as an internal control for the reagents.
Reagents And Materials Provided
1. 20 Individually packed test devices: Two test strips are contained in each device. Each strip contains colored conjugates and reactive reagents pre-coated at the corresponding regions.
2. 2 Extraction Buffer vial: 0.01 M Phosphate buffered saline (PBS) and 0.02% sodium azide.
3. 1 positive control (on request only) : Contain inactived Trichomonas vaginalis and Candida albicans. For external control.