CDIA™ Troponin I Immunofluorescence Test Cassette, Serum/Whole Blood/Plasma - Creative Diagnostics

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Cardiac Markers Tests | cTnI (troponin) |

CDIATM Troponin I Immunofluorescence Test Cassette, Serum/Whole Blood/Plasma (DTSJYJ062)

* For research use only, not for use in diagnostic procedures.

Intended Use

The CDIATM Troponin I Immunofluorescence Test Cassette is designed for quantitative determination of cardiac troponin I (cTnI) in human serum, whole blood or plasma.
  • Serum, Whole Blood, Plasma
  • Kit
  • Quantitative: Detection limit, 0.16 ng/mL; Linear rang, 0.16-32 ng/mL

Principle Of The Test

The test uses an anti-human cTnI monoclonal antibody conjugated with fluorescence latex and another anti-human cTnI monoclonal antibody coated on the test line. After the sample has been applied to the test strip, the fluorescence latex-labelled anti-human cTnI monoclonal antibody binds with the cTnI in sample and forms a marked antigen-antibody complex. This complex moves to the test card detection zone by capillary action. Then marked antigen-antibody complex is captured on the test line by the anti-human cTnI monoclonal antibody. The fluorescence intensity of the test line increases in proportion to the amount of cTnI in sample. Then insert test card into the Fluorescent Immunoassay Analyzer FIA7100, the concentration of cTnI in sample will be determined and displayed on the screen.

General Description

Cardiac troponin I (cTnI) is a cardiac muscle protein with a molecular weight of 22.5 kilodaltons. Together with troponin T (TnT) and troponin C (TnC), TnI forms a troponin complex in heart to play a fundamental role in the transmission of intracellular calcium signal actin-myosin interaction. The human cTnI has an additional amino acid residues on its N -terminal that are not exist on the skeletal forms thus making cTnI a specific marker for indicating cardiac infarction. cTnI is released rapidly into blood after the onset of acute myocardial infarction (AMI). Its release pattern is similar to CK-MB (4-6 hours after the onset of AMI). However, CK-MB level returns to normal after 36-48 hours, while levels of cTnI remains elevated for up to 6-10 days. The level of cTnI is very low in normal healthy people, and not detected in patients with skeletal muscle injury. Therefore, cTnI is a specific marker for diagnosis of AMI.This kit is intended to use in quantitative detection of cardiac troponin I content in human serum, plasma or whole blood.

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