The Tylosin Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Tylosin residue in milk.
Tylosin (MW = 916.14), is a macrolide antibiotic that is active against gram-positive and gram-negative bacteria. It is also effective in treating respiratory diseases caused by mycoplasms. It is widely used as a feed additive for growth promotion as well as therapeutically in food producing animals, including cattle, chickens and swine. Tylosin is also used for the reduction of the incidence of liver abscesses caused by sphaerophorous necrophorous. Food and Drug Administration of the United States has set the guidelines for the allowable level of tylosin in animal feeds and the specific tolerance for tylosin in edible products of animals. Tylosin EIA kit is a rapid and simple detection system for tylosin in feed extract.
Principle Of The Test
The Tylosin Rapid Test is based on competitive lateral flow immunochromatographic assay. The Tylosin conjugate in the test zone will capture the immuno-gold (colloid gold- Tylosin antibody conjugate), when there is very little dissociative Tylosin in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Tylosin is present in concentration of 50 ppb and above which explains a positive result.