CDIA^TM Xylella fastidiosa (Xf) Test Cassette (DTSPB4)

General Description

Xylella fastidiosa (Xfas) is one of the most dangerous plant pathogens that causes Pierce's disease in grapevines, phoney peach disease, bacterial leaf scorch, olive tree decline, oleander leaf scorch, and other common disease worldwide, with huge economic impact for agriculture. Xylella fastidiosa is most commonly spread through the nursery trade by insects. The best way to manage Xylella fastidiosa is to detect and remove infected plants as early as possible and to prevent the spread of its vectors.

Principle Of The Test

The development of rapid test strips is based on Lateral Flow Device (LFD) technology using specific antibodies. A LFD format consists of antibody-coated latex beads latex (or colloidal gold) which will bind the specific pathogen antigen absorbed from the plant extract. The agglutination is accumulated at a specific location by the presence of a line of target specific antibody, which immobilises the agglutinated latex whilst allowing the background reagents to be washed away by continued flow along the membrane. The membrane contains a strip of target-specific antibody, test strip and a strip of another antibody that binds to the specific antibody, control strip. Latex beads containing bound antigen are trapped on the test strip leaving a visible line. Surplus latex beads that do not contain antigen are trapped on the control strip showing that the assay has worked. This provides a non-subjective and clear read out of a positive result against a low background.