The CDIATM Zearalenone Immunofluorescence Test Strip (Feed) is a lateral flow immunoassay for the detection of zearalenone in feed.
The Test is a competitive binding immunoassay in which zearalenone in a sample compete with immobilized zearalenone conjugate for limited labeled antibody binding sites. When a sufficient amount of specimen is applied to the sample pad of the test device, the specimen migrates through the test device by capillary action. The zearalenone in the sample combines with the antibody labeled with the fluorescent microspheres, and inhibits the binding of the antibody and zearalenone on the NC membrane test line (T line) , thus resulting in changes in the fluorescence intensity of the T and C line. Read the result by using the fluorescence reader.
Zearalenone (previously known as F-2 toxin) is a nonsteroidal oestrogenic mycotoxin biosynthesized through a polyketide pathway by a variety of Fusarium fungi. Fungi-producing ZEA contaminate corn and also colonize, to a lesser extent, barley, oats, wheat, sorghum, millet and rice. In addition, the toxin has been detected in cereals products like flour, malt, soybeans and beer. Fungi of the genus Fusarium infect cereals in the field. Toxin production mainly takes place before harvesting, but may also occurs post harvest if the crop is not handled and dried properly. The CDIATM Zearalenone Immunofluorescence Test Strip (Feed) is a lateral flow immunoassay for the detection of zearalenone in feed.