Technical Notes - Creative Diagnostics

Technical NotesHome > Technical Notes

Contact Us

Technical Notes

General Technical Note

Lateral flow test strips based on the principles of immunochromatography exist for a wide array of target analytes. Its structure, components, principles and formats of lateral flow assay, labels that are used in the construction of lateral flow immunoassays (LFAs), and development steps, reader devices for LFAs are summarized.

 


 

Technical Note 1 ---- Components of Lateral Flow Tests

Strips used for LFA contain four main components: Sample pad, Conjugate pad, Absorbent Pad, Adhesive Card. In more complex designs, the strips are placed into plastic housings. The housing is used to expose the sample pad, maintain proper alignment of the materials, and indicate positions of the test and control lines. While an adhesive backing is normally used for mounting of the porous materials during the manufacturing process.

 


 

Technical Note 2 ---- Common Formats of Lateral Flow Tests

Antibodies and nucleic acids are used as biorecognition elements for developing a LFA. For antibody based LFAs, there are two formats: Sandwich Format Lateral Flow Assay, Competitive Format Lateral Flow Assay. Nucleic Acid Based Lateral Flow Assays are based on nucleic acids

 


 

Technical Note 3 ---- Labels of Lateral Flow Tests

List of materials used as a label in LFA is very vast which includes gold nanoparticles, colored latex beads, magnetic particles, carbon nanoparticles, selenium nanoparticles, silver nanoparticles, quantum dots, up converting phosphors, organic fluorophores, textile dyes, enzymes, liposomes and others

 


 

Technical Note 4 ---- Major steps in LFA

Major steps in LFA are: (1) Preparation of antibody against target analyte, (2) preparation of label, (3) Labeling of biorecognition molecules, (4) Assembling, (5) Optimizing, (6) Application of sample and obtaining results.

 


 

Technical Note 5 ---- Device reader of Lateral Flow Tests

We have provided an overview of commercially available lateral flow immunoassay readers. Several reader devices have become commercially available and these can be grouped into fluorescence, magnetic particle, and colorimetric readers. In many cases, charge-coupled device (CCD)-based image systems and scanning systems are also utilized in these readers.

 


 

Get in touch